Antibody detection of SARS-CoV spike and nucleocapsid protein

Biochem Biophys Res Commun. 2004 Feb 20;314(4):931-6. doi: 10.1016/j.bbrc.2003.12.195.

Abstract

Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Fluorescent Antibody Technique
  • Humans
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / immunology*
  • Molecular Sequence Data
  • Nucleocapsid Proteins / chemistry
  • Nucleocapsid Proteins / immunology*
  • Severe acute respiratory syndrome-related coronavirus / immunology
  • Severe acute respiratory syndrome-related coronavirus / isolation & purification*
  • Spike Glycoprotein, Coronavirus
  • Subcellular Fractions
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / immunology*

Substances

  • Membrane Glycoproteins
  • Nucleocapsid Proteins
  • Spike Glycoprotein, Coronavirus
  • Viral Envelope Proteins