The highly conserved AAA ATPase Cdc48/p97 acts on ubiquitylated substrate proteins in
cellular processes as diverse as the fusion of homotypic membranes and the degradation of …

… at 95 C, reverse transcription was performed for 15 min at 50 C and terminated by incubation
for 3 min at 95 C. Subsequently, 40 cycles of qPCR were performed (95 C for 15 s; 60 C for …

3D printing is a method of manufacturing in which materials, such as plastic or metal, are
deposited onto one another in layers to produce a three dimensional object, such as a pair of …

Context The cognitive side effects of medications with anticholinergic activity have been
documented among older adults in a variety of clinical settings. However, there has been no …

… To obtain further insight into the mutual dependence of the observed interactions, we
analysed a C-terminally truncated variant of Ubx2 lacking the UBX domain. Ubx2 ΔUBX localizes …

… was denatured by heating to 90 C for 1 min and then placed … for 1 min at 90 C and then
incubated at 37 C for 3 h, yielding … amounts of recombinant protein at 25 C for 15 min. Binding …

… UBX domains at 4 C and washed with buffer C. Cdc48 (10 μg) was incubated with the loaded
beads for 1 h at 4 C. The beads were extensively washed with buffer C, and bound Cdc48 …

10.7554/eLife.19850.001 Actin has well established functions in cellular morphogenesis.
However, it is not well understood how the various actin assemblies in a cell are kept in a …

… , were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10 or
20% fetal bovine serum (FBS) and penicillin/streptomycin, with appropriate selection at 37C in …

Schizophrenia is a highly heritable disorder. Genetic risk is conferred by a large number of
alleles, including common alleles of small effect that might be detected by genome-wide …