Development of a Multiplex PCR for the Detection of asa1, gelE, cylA, esp, and hyl Genes in Enterococci and Survey for Virulence Determinants among European Hospital …

…, T Van Autgaerden, C Vael, C Lammens… - Journal of clinical …, 2004 - Am Soc Microbiol
… An initial activation step at 95C for 15 min, during which the … (94C for 1 min), annealing
(56C for 1 min), and extension (72C for 1 min), followed by one cycle consisting of 10 min at 72C. …

[HTML][HTML] Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016

BB Xavier, C Lammens, R Ruhal… - …, 2016 - eurosurveillance.org
… 3’, and the following cycling conditions: 33 cycles of 95 C × 3 min, 65 C × 30 s, 72 C × 1
min, followed by 1 cycle of 72 C × 10 min. We found mcr-2 in 11/53 porcine and 1/52 bovine …

Effect of azithromycin and clarithromycin therapy on pharyngeal carriage of macrolide-resistant streptococci in healthy volunteers: a randomised, double-blind …

S Malhotra-Kumar, C Lammens, S Coenen… - The Lancet, 2007 - thelancet.com
… S Malhotra-Kumar and C Lammens did the sampling and experimental work. S Coenen
and K Van Herck did the statistical analysis. All authors saw and approved the final version of …

Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human …

P Descheemaeker, C Lammens… - … of Systematic and …, 1997 - microbiologyresearch.org
… for 5 min, and elongation at 72C for 5 min, followed by 30 … 94C for 1 min, annealing at 55C
for 1 min, and elongation at 72C for 2 min, followed by one cycle consisting of 10 min at 72C. …

[HTML][HTML] Aetiology of lower respiratory tract infection in adults in primary care: a prospective study in 11 European countries

M Ieven, S Coenen, K Loens, C Lammens… - Clinical microbiology …, 2018 - Elsevier
… using direct microscopy to assess the quality (ratio of white blood cells/epithelial cells ≥1
as criterion for good quality), then Gram-stained, cultured and subsequently frozen at −80C. …

Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium

…, L De Zutter, JM Dumont, C Lammens… - Journal of …, 2001 - academic.oup.com
… coli than with C. jejuni, and several studies have emphasized that C. coli isolates are
more likely to acquire antibiotic resistance than are C. jejuni isolates. In this study, rates of …

Multiplex PCR for simultaneous detection of macrolide and tetracycline resistance determinants in streptococci

S Malhotra-Kumar, C Lammens… - Antimicrobial agents …, 2005 - Am Soc Microbiol
C, 30 cycles of 1 min of denaturation at 93C, 1 min of annealing at 62C, and 4 min of extension
at 65C, followed by one cycle of 3 min of elongation at 65C. PCR products were analyzed …

[HTML][HTML] Colistin resistance gene mcr-1 harboured on a multidrug resistant plasmid

…, BB Xavier, AJ Das, C Lammens… - The Lancet infectious …, 2016 - thelancet.com
Yi-Yun Liu and colleagues reported, for the first time, plasmid-mediated colistin resistance
in Escherichia coli isolated from animals, food, and patients in China. 1 These data bring to …

Use of serum C reactive protein and procalcitonin concentrations in addition to symptoms and signs to predict pneumonia in patients presenting to primary care with …

SF van Vugt, BDL Broekhuizen, C Lammens… - Bmj, 2013 - bmj.com
RESEARCH Page 1 Use of serum C reactive protein and procalcitonin concentrations in
addition to symptoms and signs to predict pneumonia in patients presenting to primary care …

Colistin-resistant Escherichia coli harbouring mcr-1 isolated from food animals in Hanoi, Vietnam

…, BB Xavier, AJ Das, C Lammens… - The Lancet infectious …, 2016 - thelancet.com
… T>C IHD86_4 … *Surbhi Malhotra-Kumar, Basil Britto Xavier, Anupam J Das, Christine
Lammens, Ha Thi Thu Hoang, Ngoc Thi Pham, Herman Goossens … C