… In order to achieve as broad a picture as possible and to ensure M Harford-Cross, … M
Harford-Cross … Address for correspondence Dr M Harford-Cross, Ashfield House, Kirkby Malzeard, …

The styrene oxidation activity of cytochrome P450 cam has been greatly improved by rational
protein engineering. Compared to the wild-type enzyme, the active-site mutants Y96A and …

Mutations of the active site residues F87 and Y96 greatly enhanced the activity of cytochrome
P450 cam (CYP101) from Pseudomonas putida for the oxidation of the polycyclic aromatic …

The protein engineering of CYP enzymes for structure–activity studies and the oxidation of
unnatural substrates for biotechnological applications will be greatly facilitated by the …

Mutants of the heme monooxygenase cytochrome P450 cam in which Y96 had been replaced
with hydrophobic residues, have been shown to oxidise naphthalene and pyrene with …

… The protein was equilibrated into 50 mm … m with the addition of 200 mm KCl or 1 mm camphor
if necessary. For camphor binding titrations, aliquots of a 2 mm camphor solution in 50 mm …

He and Margaret retired to the Isle of Mull, where he was much sought after as a locum. After
the death of his wife, who supported him throughout his career, he moved to be with his …

Agrocybe aegerita peroxidase (AaP) is a versatile heme-thiolate protein that can act as a
peroxygenase and catalyzes, among other reactions, the hydroxylation of aromatic rings. This …

… a fiber loop and soaked in 100 mm MES buffer, pH 6.5, 100 mm sodium acetate, 100 mm
KCl, 20% PEG8000, and 100 μm1,3,5-TCB (added as a 100 mm stock in ethanol) for 5 days. …

Oxidations are key reactions in chemical syntheses. Biooxidations using fermentation processes
have already conquered some niches in industrial oxidation processes since they allow …